Application

Mouse anti-Rabbit light chain Antibody, biotin conjugate is an antibody against Rabbit light chain for use in ELISA, FC, WB, IH.

Western Blotting: 1:20,000 - 1:400,000
Immunohistochemistry: 1:500 - 1:5,000 on tissue sections
Flow Cytometry: 1:200 - 1:1,000

Optimal working dilutions must be determined by the end user.

General description

Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and gamma light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized from 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 0.05% Sodium Azide. 15 mg/mL BSA as stabilizer (IgG-Free, Protease-Free)

Specificity

Minimal cross-reaction with bovine, goat, Armenian hamster, horse, human, mouse, rat, and sheep.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1.1 mg/mL). For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Target description

25 kDa